Members: Brian, Eunice, Thomas, Reylene
The two types of fungi observed in this group was Shiitake from the basidiomycota and Pilobolus from zygomycota. Although they are both eukaryotes and fungus, they have their own funky names after them:
Fungus Name
|
Shiitake
|
Pilobolus
|
Domain
|
Eukaryote
|
Eukaryote
|
Kingdom
|
Fungi
|
Fungi
|
Division
|
Basidiomycota
|
Zygomycota
|
Class
|
Agaricomycetes
|
Mucoromycotina
|
Order
|
Agaricales
|
Mucorales
|
Family
|
Marasmiaceae
|
Pilobolaceae
|
Genus
|
Lentinula
|
Pilobolus
|
Species
|
L. edodes
|
Similarities
- Chitin cell walls
- Has dikaryotic stage
- Underground hyphae filaments
- Lack chloroplasts(no photosynthesis)
Shiitake(Basidiomycota)
- Wood decomposers(remember lignin peroxidase?)
- Produce fruiting body(mushroom)
- Let spores out through gills
- Hyphae has septa(cross walls)=mycelia
Pilobolus(Zygomycota)
- Hyphae lacks septa
- Hyphae fuse to make zygosporangia
- Shoot spores out of sporangium
If you are allergic to letters, look at the pictures below.
Life cycle of Shiitake, basidiomycota:
Lifecycle of Pilobolus, zygomycota:
For experiment methods, Pilobolus culture dish was set inside a milkshake cup with a hole on top to let in sunlight. Shiitake log was covered with a transparent plastic bag and sprayed with water as often as possible. For more details, reference to the EdCC BIOL 213 lab manual.
Few weeks later, while Pilobolus was happily enjoying their life on feces, shooting spores towards the sun light, Shiitake seemed like it wasn't enjoying it's log at all.
spores!
Mushroom... crumbs?
Shiitake fungi normally grow in warm moist climates of Southeast Asia, so maybe it was home sick.
But eventually it opened it's fruiting body by our love and DI water.
And was picked and dissected for slides!
Mushroom sibling rivalry
Would definitely recommend this assignment to future college students short in cash and vitamin D. Clinical trial kind of demonstrated. We assure no animals or our group members were harmed during the trial.
That is interesting that your mushroom culture ended up with a fruiting body. my group ended up doing the same thing roughly but nothing grew. I also like the drawings that you made of the life cycles on the pilobolus and shitake mushroom.
ReplyDeleteGwen, here is my post!
DeleteHi Fun-Guys team, your post is very interesting and very clear in terms of both text and illustrations. I also agree that shiitake mushroom must be in grown in an environment that is hot and humid like Southeast Asia. However, you all did a great job since you managed to successfully grow some mushroom fruiting bodies.
ReplyDeleteMoreover, I like the way you draw life cycles on those images. This shows a very clear understanding of the fungi life cycle. Thank you for sharing this with us.
Wow! I'm amazed you guys managed to keep the mushroom growing. Our mushroom (pearl oyster) died on the third week although it does not have to be in a warm or cold environment. It would be nicer if you guys had dissected the mushroom and looked it through the microscope to see more interesting features.
ReplyDeleteHi Fun-Guys,
ReplyDeleteI appreciate you you combine humor, artistry, and information. The way you broke down the differences between Pilobolus and Shiitake was informative to me. One thing I notice about your Pilobolus culture is that the spores do not seem to be very directional. My group also experienced this. We were expecting most of the spores to land on the piece of tape covering the light hole. Instead, our results were more like yours. My theory is that maybe we made the light hole too big or that the cardboard structure was too tall, or maybe a combination of both. What do you think?
Amy