Mystery TSA Dish Bacteria
by Jaena Ristel Bautista
DAY 0
Figure 1: Day 0
Left plate: dish at 37º C
Right plate: dish at room temperature
Before starting this experiment, I was hoping to grow a lot of bacteria on my plates. I was curious how much bacteria there can be on things that we often touch, in and out of the classroom and even the soles of our shoes. This is why I came up swabbing things I know could grow a lot of bacteria: soles of shoes, edge of the table, sink, and plant pot in the lab.
DAY 2
Figure 2.1: Day 2
dish at room temperature
Figure 2.2: Day 2
dish at 37ºC
On second day of observation, results are already seen on my dish. Both plates can be seen of colonies of bacteria except for the table maybe because of disinfecting it regularly (which is good! Because that would be gross!). The plant pot has the largest number of bacterial growth and next would be the shoes. As seen in the pictures, more colonies of bacteria showed up in the dish incubated at 37ºC. I assume that this is because species of bacteria from my samples are able to adapt more at higher temperature (37C) than at room temperature.
Colonies:
dish at room temperature
Shoes: ~600
Plant Pot: almost lawn
Sink: 7
Table: none
dish at 37ºC
Shoes: almost lawn
Plant Pot: almost lawn
Sink: 11
Table: none
DAY 7
Figure 3.1: Day 7
On the last day of observation, bacterial growth had grown larger. It was fascinating to see that even the table section has appeared some cottony/thread-like species of bacteria. Now, both dishes almost have the same number of colonies and almost all samples grew lawns. I assume that if both plates are left longer, I wouldn't be able to identify clearly which bacteria is which due to increasing growth. Observing the two dishes, types of these bacteria are capable of reproducing in both temperatures!
CLOSER IMAGE OF DISHES
Figure 3.2
Day 7: Room Temperature
The bacteria that grew on the sink section (pointed) of my plate is really interesting. I am trying to identify this kind but it is really difficult to know because it could be anything plus there are thousand kinds of bacteria out there. And I could be wrong that is still a bacteria. Gwen mentioned that it looks like somewhat-kind of a fungi. If you look at the picture closely, you can see that it grew upward.
Figure 3.3
Day 7: 37ºC
Colonies:
dish at room temperature
Shoes: ~700
Plant Pot: lawn
Sink: lawn
Table: lawn
dish at 37ºC
Shoes: almost lawn
Plant Pot: almost lawn
Sink: lawn
Table: lawn
I found it very cool that you can see the large amounts of growth on the sink slide. In the very beginning it had little growth on both plates, but as time went on, the sink sample started to grow quickly and farther out. It is also different than other ones I've seen on other people's post that it looks like one large lawn instead of many smaller ones. Seeing from earlier pictures there smaller individual ones but on the last day you can't even see the individual samples. Good job on such a variety of locations!
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